microfluidic device standard neuron device cat Search Results


microfluidic devices xona microfluidics cat#snd450  (Xona Microfluidics)
90
Xona Microfluidics microfluidic devices xona microfluidics cat#snd450
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Microfluidic Devices Xona Microfluidics Cat#Snd450, supplied by Xona Microfluidics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
microfluidic devices xona microfluidics cat#snd450 - by Bioz Stars, 2026-04
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microfluidic chips  (fluidigm)
96
fluidigm microfluidic chips
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Microfluidic Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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microfluor 1 white flat-bottom plate  (Thermo Fisher)
90
Thermo Fisher microfluor 1 white flat-bottom plate
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Microfluor 1 White Flat Bottom Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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microfluidic chamber rd450  (Xona Microfluidics)
90
Xona Microfluidics microfluidic chamber rd450
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Microfluidic Chamber Rd450, supplied by Xona Microfluidics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superfrost® plus microslides  (Avantor)
90
Avantor superfrost® plus microslides
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Superfrost® Plus Microslides, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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green polyethylene microslide plastic boxes  (Fisher Scientific)
90
Fisher Scientific green polyethylene microslide plastic boxes
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Green Polyethylene Microslide Plastic Boxes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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microfluidic plate  (Millipore)
90
Millipore microfluidic plate
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Microfluidic Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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compartmentalised microfluidic devices mfd  (Xona Microfluidics)
90
Xona Microfluidics compartmentalised microfluidic devices mfd
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Compartmentalised Microfluidic Devices Mfd, supplied by Xona Microfluidics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ella microfluidics platform  (Bio-Techne corporation)
86
Bio-Techne corporation ella microfluidics platform
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Ella Microfluidics Platform, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microfluor 2 u-bottom black 96-well plates  (DYNEX tech)
90
DYNEX tech microfluor 2 u-bottom black 96-well plates
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Microfluor 2 U Bottom Black 96 Well Plates, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glass slides extra thick microslides  (Fisher Scientific)
90
Fisher Scientific glass slides extra thick microslides
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Glass Slides Extra Thick Microslides, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellasic onix2 microfluidic platform  (Millipore)
90
Millipore cellasic onix2 microfluidic platform
Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of <t>microfluidic</t> device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel
Cellasic Onix2 Microfluidic Platform, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of microfluidic device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel

Journal: Journal of Neurovirology

Article Title: SARS-CoV and SARS-CoV-2 display limited neuronal infection and lack the ability to transmit within synaptically connected axons in stem cell–derived human neurons

doi: 10.1007/s13365-023-01187-3

Figure Lengend Snippet: Sarbecovirus infection of ex vivo model of interconnected human neural network to assess trans-synaptic transmission. A Stem cell–derived NPCs were seeded in both panels of microfluidic device separated by microchannels which allows only the neurites to pass through. After seeding, NPCs were differentiated for 20 (RABV) to 21 days (sarbecoviruses) to generate neurons in both panels interconnected by neurites through the microchannels. The neuronal network is identified by TUJ1 staining (red) and F-actin stain to visualise the neural architecture (magenta). The ex vivo models were infected MOI 1 with RABV for 48 h, which served as a positive control for trans-synaptic transmission ( B ) or different strains of sarbecoviruses ( C, D, E ) at for 24 h. To prevent passive diffusion of virus from the infected panel into the non-infected panel, a higher volume of media is maintained in the non-infected panel. After 24 h of infection, neural cultures were fixed, stained, and imaged in the microfluidic device. Tile images were taken with 20 × objective with Z-stacks and stitched together. Image B shows trans-synaptic spread of RABV from the infected to non-infected panel identified by staining with a rabies anti-nucleoprotein antibody (green). Images C, D, and E show SARS and SARS-CoV-2 infections stained with anti-SARS2-S1 antibody (green) in a subset of neurons in the infected panel (boxed area) but viral antigen was not observed in the non-infected panel

Article Snippet: Xona microfluidic devices (Xona Microfluidics, Cat#SND450) were sterilised and plasma bonded to glass coverslips (24 × 40 mm; Menzel Glaser) using a plasma cleaner (PDC-32G-2, Harrick Plasma).

Techniques: Infection, Ex Vivo, Transmission Assay, Derivative Assay, Staining, Positive Control, Diffusion-based Assay, Virus